Data for the Constructed Mutant: YYCHd

General Information

Name of the MutantYYCHd (disruption)
Used VectorpMutinT3
Used Enzyme SitesBglII
Gene Size1365 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYCHd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) FKF003: cgaggcagatctCGATATTACTTACGGTAC
Reverse Primer:(cgaggcagatctgaattc tag) FKR003: cgaggcagatctgaattcGTATTCAAACGACTGATTAG
Length of the Cloned Region: 23 to 447 Length: 425
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYCHd