Data for the Constructed Mutant: YYCGp

General Information

Name of the MutantYYCGp (promoter fusion)
Used VectorpMutinT3
Used Enzyme SitesBglII
Gene Size1833 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYCGp (promoter fusion)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) FKF012: cgaggcagatctGAGAAACCCAGAACAGGAC
Reverse Primer:(cgaggcagatctgaattc tag) FKR002: cgaggcagatctgaattcCATACTCGCGACAACGTAG
Length of the Cloned Region: -28 to 510 Length: 539
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYCGp