Data for the Constructed Mutant: YBANd

General Information

Name of the MutantYBANd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size762 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:

Mutant Construction for YBANd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(ggaagc tag) ybaN-F: ggaagcTTATATCCAAAGAGCTGTCC
Reverse Primer:(ttggat tag) ybaN-R: ttggatCCGGTAGGGGGTCTTAATAG
Length of the Cloned Region: 87 to 473 Length: 387
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBANd