Data for the Constructed Mutant: YXBGd

General Information

Name of the MutantYXBGd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size786 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXBGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxbGH: GCCGaagcttAACAGCAGTTATCACAGGCG
Reverse Primer:(agatct tag) yxbGBG: GCGCagatctAGGTCAACCGGATATTCGTG
Length of the Cloned Region: 21 to 320 Length: 300
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXBGd