Data for the Constructed Mutant: YXBAd

General Information

Name of the MutantYXBAd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size267 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXBAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxbAH: GCCGaagcttAATCCAGAAATGCCATCAAG
Reverse Primer:(agatct tag) yxbABG: GCGCagatctCATGTATGCTGACCTTGCCG
Length of the Cloned Region: 31 to 148 Length: 118
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXBAd