Data for the Constructed Mutant: YCCKd

General Information

Name of the MutantYCCKd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size930 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCCKd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaacgtt tag) yccK-1: gcgcaacgttAACGAAGCTGAAGGTGAAGC
Reverse Primer:(gccgagatgt tag) yccK-2: gccgagatgtTCTGTTTTCAGCCGCTTCAG
Length of the Cloned Region: 27 to 356 Length: 330
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCCKd