Data for the Constructed Mutant: RIPXd
General Information
Name of the Mutant
RIPXd (disruption)
Host
Used Vector
pMutinT3
Used Enzyme Sites
HindIII and BamHI
Gene Size
888 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
no
Mutant Submission to NIG:
yes
Correspondence:
Mutant Construction for RIPXd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
aagaagctt
tag)
ripX-F:
aagaagcttGATCAAATAAAGGATTTCATC
Reverse Primer:
(
taatacgactcactatagggcgaggatcc
tag)
ripX-R:
taatacgactcactatagggcgaggatccCCTGAGCAGAAATTGATG
Length of the Cloned Region: 7 to 261 Length: 255
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
RIPXd