Data for the Constructed Mutant: MTLAd

General Information

Name of the MutantMTLAd (disruption)
Host168
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1830 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for MTLAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) mtlA1: gcgcaagcttGCAAGGCGGCATGAAAGTGA
Reverse Primer:(gccgagatct tag) mtlA2: gccgagatctCCAAGCGGACCCATAATCAT
Length of the Cloned Region: 21 to 338 Length: 318
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of MTLAd