Data for the Constructed Mutant: YCKFd

General Information

Name of the MutantYCKFd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size555 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YCKFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yckF1: gcgcaagcttGGAATTGTTGCCGCTTTAAC
Reverse Primer:(gccgagatct tag) yckF2: gccgagatctCCTTTACGATTTGCAGGTCA
Length of the Cloned Region: 316 to 661 Length: 346
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCKFd