Data for the Constructed Mutant: YCKEd

General Information

Name of the MutantYCKEd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1431 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YCKEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yckE1: gcgcaagcttAGCATCCAGAATCTTTTCCG
Reverse Primer:(gccgagatct tag) yckE2: gccgagatctCTCGATGTGATGAGAAAGCA
Length of the Cloned Region: 11 to 351 Length: 341
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCKEd