Data for the Constructed Mutant: YCKCd

General Information

Name of the MutantYCKCd (disruption)
Host168
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size453 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no
Correspondence:


Mutant Construction for YCKCd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yckC1: gcgcaagcttAAACCAGCCGGATTTTGGAT
Reverse Primer:(gccgagatct tag) yckC2: gccgagatctTCGTACGGCAATCAGAATCA
Length of the Cloned Region: 13 to 348 Length: 336
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YCKCd