Data for the Constructed Mutant: YCKBd

General Information

Name of the MutantYCKBd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size861 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YCKBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yckB1: gcgcaagcttGAATGACATCGATGTGACGA
Reverse Primer:(gccgagatct tag) yckB2: gccgagatctTTGTAATGTTGAGATCCGGG
Length of the Cloned Region: 336 to 664 Length: 329
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCKBd