Data for the Constructed Mutant: YCKAd

General Information

Name of the MutantYCKAd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size678 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCKAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yckA1: gcgcaagcttATTTTCAACACGAAGCTGGC
Reverse Primer:(gccgagatct tag) yckA2: gccgagatctTTTCGACGGATGAAATGGCT
Length of the Cloned Region: 28 to 370 Length: 343
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCKAd