Data for the Constructed Mutant: YCBUd

General Information

Name of the MutantYCBUd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size1110 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCBUd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycbU-1: gcgcaagcttATCGCCAGTTATTCCCAACC
Reverse Primer:(gccgagatct tag) ycbU-2: gccgagatctTACGGTATAATCGGAGTGTG
Length of the Cloned Region: 23 to 369 Length: 347
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCBUd