Data for the Constructed Mutant: YCBNd

General Information

Name of the MutantYCBNd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size837 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCBNd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycbN-1: gcgcaagcttGAAATCTATGGCTTTCTCGG
Reverse Primer:(gccgagatct tag) ycbN-2: gccgagatctCATTCACCGGCTCATCCAGA
Length of the Cloned Region: 27 to 424 Length: 398
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCBNd