Data for the Constructed Mutant: YCBMd

General Information

Name of the MutantYCBMd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size474 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCBMd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycbM-1: gcgcaagcttCGCTGTCATCGCTCTGGCGT
Reverse Primer:(gccgagatct tag) ycbM-2: gccgagatctCTTTGAGCGCATGATCGTCT
Length of the Cloned Region: 21 to 178 Length: 158
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCBMd