Data for the Constructed Mutant: YCBLd

General Information

Name of the MutantYCBLd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size678 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCBLd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycbL-1: gcgcaagcttAGACGACCACTCAATCAGTG
Reverse Primer:(gccgagatct tag) ycbL-2: gccgagatctCTCCTCTGCCGAATATTGCG
Length of the Cloned Region: 12 to 366 Length: 355
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCBLd