Data for the Constructed Mutant: YCBHd

General Information

Name of the MutantYCBHd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size1530 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCBHd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycbH-1: gcgcaagcttAAGAACCAAGCCCCCCTTTA
Reverse Primer:(gccgagatct tag) ycbH-2: gccgagatctTCCGCATTTCGGTATCCTTC
Length of the Cloned Region: 19 to 374 Length: 356
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCBHd