Data for the Constructed Mutant: YCBGd

General Information

Name of the MutantYCBGd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size699 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCBGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycbG-1: gcgcaagcttAAGTCGATACAGTCAATCGG
Reverse Primer:(gccgagatct tag) ycbG-2: gccgagatctTTTCCGCGGCAATTGTCACG
Length of the Cloned Region: 26 to 364 Length: 339
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCBGd