Data for the Constructed Mutant: YCBFd

General Information

Name of the MutantYCBFd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size1365 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCBFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycbF-1: gcgcaagcttTGAAGGTGATTCCTGTTGCG
Reverse Primer:(gccgagatgt tag) ycbF-2: gccgagatgtCGACCGGTTCCTGAAGAAAC
Length of the Cloned Region: 68 to 427 Length: 360
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCBFd