Data for the Constructed Mutant: YXJFd

General Information

Name of the MutantYXJFd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size771 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXJFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxjFH: GCCGaagcttGCAGAAGAAGGCTTTGACGC
Reverse Primer:(agatct tag) yxjFBG: GCGCagatctGCCCACTAATCCATTAACAG
Length of the Cloned Region: 142 to 435 Length: 294
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXJFd