Data for the Constructed Mutant: YXJEd

General Information

Name of the MutantYXJEd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size648 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXJEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxjEH: GCCGaagcttAATGGTCAAACGGGCTGTAC
Reverse Primer:(agatct tag) yxjEBG: GCGCagatctCGCCGAGAATAGCTAAATCG
Length of the Cloned Region: 21 to 310 Length: 290
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXJEd