Data for the Constructed Mutant: YXJAd

General Information

Name of the MutantYXJAd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size1191 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXJAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxjAH: GCCGaagcttCGGTCTCATTGTGATTATGG
Reverse Primer:(agatct tag) yxjABG: GCGCagatctTAATTTCGGCAAGCGGGAAG
Length of the Cloned Region: 24 to 399 Length: 376
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXJAd