Data for the Constructed Mutant: YXITd

General Information

Name of the MutantYXITd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size240 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXITd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxiTH: GCCGaagcttCGCGGGCATTGCAGTCTTGC
Reverse Primer:(agatct tag) yxiTBG: GCGCagatctCTTGTGGATACGTTAACTCC
Length of the Cloned Region: 27 to 157 Length: 131
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXITd