Data for the Constructed Mutant: YXISd

General Information

Name of the MutantYXISd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size288 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXISd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxiSH: GCCGaagcttTGAAGCTTACGAACGCGATG
Reverse Primer:(agatct tag) yxiSBG: GCGCagatctTCATTCAGCTTTTGCTGCGG
Length of the Cloned Region: 21 to 140 Length: 120
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXISd