Data for the Constructed Mutant: YXIQd

General Information

Name of the MutantYXIQd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1278 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXIQd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxiQH: GCCGaagcttCGAGATGATGATTCAGGGCG
Reverse Primer:(agatct tag) yxiQBG: GCCGagatctCGAACCAAGCATCGCTATTC
Length of the Cloned Region: 138 to 441 Length: 304
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXIQd