Data for the Constructed Mutant: YXIOd

General Information

Name of the MutantYXIOd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size1284 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXIOd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxiOH: GCCGaagcttGCTGGGTGTTATATGACTGG
Reverse Primer:(agatct tag) yxiOBG: GCGCagatctAGCAGCCAATGTTCACTAGG
Length of the Cloned Region: 32 to 326 Length: 295
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXIOd