Data for the Constructed Mutant: YXIJd

General Information

Name of the MutantYXIJd (disruption)
Host168
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size294 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YXIJd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxiJH: GCCGaagcttAAAAAGACAGCGAGAGCTTG
Reverse Primer:(agatct tag) yxiJBG: GCGCagatctCAGCACATAATCCAAACTGC
Length of the Cloned Region: 18 to 129 Length: 112
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YXIJd