Data for the Constructed Mutant: YXIFd

General Information

Name of the MutantYXIFd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size468 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXIFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxiFH: GCCGaagcttCTCGCCGAGAATAAAAGACG
Reverse Primer:(agatct tag) yxiFBG: GCGCagatctCAAGCAATTGCCTGCTTTGC
Length of the Cloned Region: 25 to 142 Length: 118
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXIFd