Data for the Constructed Mutant: YXIEd

General Information

Name of the MutantYXIEd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size444 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXIEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxiEH: GCCGaagcttCGATTGACGGATCAGACATG
Reverse Primer:(agatct tag) yxiEBG: GCGCagatctAAGTGTTCAGGCACATAGAC
Length of the Cloned Region: 23 to 176 Length: 154
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXIEd