Data for the Constructed Mutant: YXICd

General Information

Name of the MutantYXICd (disruption)
Host168
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size267 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YXICd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxiCH: GCCGaagcttGGTGTATGACACAGTGAAGC
Reverse Primer:(agatct tag) yxiCBG: GCGCagatctTGCTCGATGGACTTCACAAC
Length of the Cloned Region: 21 to 140 Length: 120
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YXICd