Data for the Constructed Mutant: YXIBd

General Information

Name of the MutantYXIBd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size363 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXIBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxiBH: GCCGaagcttTACATTCGGTAAAAGCCGAC
Reverse Primer:(agatct tag) yxiBBG: GCGCagatctCATGTGTTCCTCTCCATGCC
Length of the Cloned Region: 20 to 178 Length: 159
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXIBd