Data for the Constructed Mutant: IOLJd

General Information

Name of the MutantIOLJd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size870 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for IOLJd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) iolJH: GCCGaagcttGCTTCTTGAAGATGCAAAGC
Reverse Primer:(agatct tag) iolJBG: GCGCagatctATATTCTCGTCAATCGGCTG
Length of the Cloned Region: 24 to 350 Length: 327
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of IOLJd