Data for the Constructed Mutant: IOLId

General Information

Name of the MutantIOLId (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size834 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for IOLId (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) iolIH: GCCGaagcttGCGACAACATTGGAAAACTC
Reverse Primer:(agatct tag) iolIBG: GCGCagatctACAATCTTCTGCTCTGTCAC
Length of the Cloned Region: 22 to 338 Length: 317
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of IOLId