Data for the Constructed Mutant: IOLCd

General Information

Name of the MutantIOLCd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size975 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for IOLCd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) iolCH: GCCGaagcttTGATATTGTTGCCATCGGCC
Reverse Primer:(agatct tag) iolCBG: GCGCagatctCGCTGCCGATCGCGATATTC
Length of the Cloned Region: 33 to 160 Length: 128
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of IOLCd