Data for the Constructed Mutant: YXAJd

General Information

Name of the MutantYXAJd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size426 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXAJd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxaJH: GCCGaagcttGCGATCATGACAACCATTTC
Reverse Primer:(agatct tag) yxaJBG: GCGCagatctACAAGACAAGGATCATACCG
Length of the Cloned Region: 22 to 169 Length: 148
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXAJd