Data for the Constructed Mutant: YXABd
General Information
Name of the Mutant
YXABd (disruption)
Host
168
Used Vector
pMutin2
Used Enzyme Sites
HindIII and BamHI
Gene Size
951 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
yes
Mutant Submission to NIG:
yes
Correspondence:
Mutant Construction for YXABd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
aagctt
tag)
yxaBH:
GCCGaagcttAAGGCATTGCGCCAAATGTG
Reverse Primer:
(
agatct
tag)
yxaBBG:
GCGCagatctAGACCTTTCTTCTGTCTTCC
Length of the Cloned Region: 38 to 334 Length: 297
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR: yes
Primer sets used for the check
No.
Primer set
Length of PCR product
1
Forward Primer
yxaBup
tgtaaaacgacggccagtGGAGGACGCAGATATGACTG
Reverse Primer
yxaBdn
caggaaacagctatgaccATAAACGGATTGTGGCAGAG
Digestion of the PCR product: yes
Enzyme used: EcoRV
Figure
Comment: ;.
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
YXABd