Data for the Constructed Mutant: YXABd

General Information

Name of the MutantYXABd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size951 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXABd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxaBH: GCCGaagcttAAGGCATTGCGCCAAATGTG
Reverse Primer:(agatct tag) yxaBBG: GCGCagatctAGACCTTTCTTCTGTCTTCC
Length of the Cloned Region: 38 to 334 Length: 297
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXABd