Data for the Constructed Mutant: YYXAd
General Information
Name of the Mutant
YYXAd (disruption)
Host
Used Vector
pMutinT3
Used Enzyme Sites
BglII
Gene Size
1200 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
no
Mutant Submission to NIG:
no
Correspondence:
Mutant Construction for YYXAd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
cgaggcagatct
tag)
FKF006:
cgaggcagatctACGAACGTGAGGAAGAAC
Reverse Primer:
(
cgaggcagatctgaattc
tag)
FKR006:
cgaggcagatctgaattcCCCAGACTTCACTTTATCTGA
Length of the Cloned Region: 11 to 555 Length: 545
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
YYXAd