Data for the Constructed Mutant: YYXAd

General Information

Name of the MutantYYXAd (disruption)
Used VectorpMutinT3
Used Enzyme SitesBglII
Gene Size1200 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYXAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) FKF006: cgaggcagatctACGAACGTGAGGAAGAAC
Reverse Primer:(cgaggcagatctgaattc tag) FKR006: cgaggcagatctgaattcCCCAGACTTCACTTTATCTGA
Length of the Cloned Region: 11 to 555 Length: 545
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYXAd