Data for the Constructed Mutant: TRERd

General Information

Name of the MutantTRERd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size714 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for TRERd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) treRHF: gccgaagcttCGCACAGCAAATTGAAGG
Reverse Primer:(gcgcggatcc tag) treRBR: gcgcggatccTCTTTGCCAAGCGTTTGC
Length of the Cloned Region: 39 to 281 Length: 243
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of TRERd