Data for the Constructed Mutant: PELd

General Information

Name of the MutantPELd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1260 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for PELd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) PE-1H: gccgaagcttGGATCAAAAGCATCCTC
Reverse Primer:(cgcggatcc tag) PE-2B: cgcggatccTTTGCAGGGATATCCAC
Length of the Cloned Region: 133 to 446 Length: 314
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of PELd