Data for the Constructed Mutant: YBBDd

General Information

Name of the MutantYBBDd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1926 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YBBDd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(ggaagct tag) ybbD-F: ggaagctTGGGCGCAGCCAGAAGCAGA
Reverse Primer:(ttggat tag) ybbD-R: ttggatCCATGGGAAACGAGCGGCAG
Length of the Cloned Region: 431 to 728 Length: 298
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBBDd