Data for the Constructed Mutant: YXXFd

General Information

Name of the MutantYXXFd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size933 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXXFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxxFH: GCCGaagcttTCTCAGAGCATACCAATCTG
Reverse Primer:(agatct tag) yxxFBG: GCGCagatctATGGAGCTGACGGAGATCAC
Length of the Cloned Region: 95 to 404 Length: 310
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXXFd