Data for the Constructed Mutant: GNTZd

General Information

Name of the MutantGNTZd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size1404 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for GNTZd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) gntZH: GCCGaagcttCATAGGCTTAGGCGTAATGG
Reverse Primer:(agatct tag) gntZBG: GCGCagatctCTGTGTCTTCATAGTGGGAG
Length of the Cloned Region: 21 to 322 Length: 302
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of GNTZd