Data for the Constructed Mutant: YWAAd

General Information

Name of the MutantYWAAd (disruption)
Host168
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size1089 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YWAAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) ywaAH: GCCGaagcttACCGAAACCAGACCCAAATC
Reverse Primer:(agatct tag) ywaABG: GCGCagatctAAGCCTTCAAGAACCTGTTC
Length of the Cloned Region: 45 to 350 Length: 306
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YWAAd