Data for the Constructed Mutant: YQXCd

General Information

Name of the MutantYQXCd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size807 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YQXCd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yqxC-F: aagaagcttACGTCAAAGAAAGAACGATTAG
Reverse Primer:(taatacgactcactatagggcgaggatcc tag) yqxC-T7R: taatacgactcactatagggcgaggatccTCCAGGCTTGTCCAAGCG
Length of the Cloned Region: 4 to 132 Length: 129
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YQXCd