Data for the Constructed Mutant: YCXDd

General Information

Name of the MutantYCXDd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size1332 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCXDd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycxD-1: gcgcaagcttTAACGAGGATAGAGGAGATG
Reverse Primer:(gccgagctct tag) ycxD-2: gccgagctctCCTTTGGCGTCCCATAAATA
Length of the Cloned Region: 23 to 361 Length: 339
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCXDd