Data for the Constructed Mutant: YCXCd

General Information

Name of the MutantYCXCd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size936 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YCXCd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycxC1: gcgcaagcttAGCAAACCCCCGCTTATATA
Reverse Primer:(gccgagatct tag) ycxC2: gccgagatctAAACATAAGCGGCAAAGACC
Length of the Cloned Region: 14 to 352 Length: 339
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCXCd