Data for the Constructed Mutant: YCXBd

General Information

Name of the MutantYCXBd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size555 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCXBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycxB1: gcgcaagcttTTTGCGCCATATTTGCTGCA
Reverse Primer:(gccgagatct tag) ycxB2: gccgagatctGATCGCAATATATTGGCCAC
Length of the Cloned Region: 116 to 453 Length: 338
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCXBd