Data for the Constructed Mutant: YACLd

General Information

Name of the MutantYACLd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1098 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YACLd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaag tag) yacL-F: aagaagCTTCATTATTTTTGGCGGCG
Reverse Primer:(ggagga tag) yacL-R: ggaggaTCCAAATAACAAATCTGGAAC
Length of the Cloned Region: 27 to 252 Length: 226
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YACLd