Data for the Constructed Mutant: CLPCd

General Information

Name of the MutantCLPCd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BglII
Gene Size2430 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for CLPCd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgataagctt tag) mecB-F: cgataagcttCTGGCGCTAGCACAGGAAGAAGC
Reverse Primer:(cgtaagatct tag) mecB-R: cgtaagatctGTTCCTGCCGCTGATGATCCTG
Length of the Cloned Region: 40 to 461 Length: 422
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of CLPCd